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KMID : 0616120170480030232
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Korean Journal of Pharmacognosy
2017 Volume.48 No. 3 p.232 ~ p.236
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Simultaneous Determination of Gastrodin and Gastrodigenin in Gastrodia elata by HPLC-UV
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Kang Kyo-Bin
Jun Jin-Bum
Yoo Gui-Jae
Kwon Eun-A
Yoon Jun-Won
Kang Byeong-Cheol
Kim Seung-Hyun
Sung Sang-Hyun
Jang Young-Pyo
Yang Hee-Jung
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Abstract
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Gastrodia elata has been widely used as a traditional medicinal herb. However, in Korean Pharmacopeia, neither a marker constituent nor an analytical procedure for G. elata has been established. In this study, we suggest gastrodin and gastrodigenin as marker constituents of G. elata, and propose an analytical procedure for simultaneous quantification of these constituents by high-performance liquid chromatography-ultraviolet spectroscopy (HPLC-UV). The analytical method was validated for its linearity, precision, accuracy, and specificity. Based on the validated method, gastrodin and gastrodigenin in 14 commercial G. elata samples were quantified.
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KEYWORD
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HPLC analysis , Gastrodia elata , Gastrodin , Gastrodigenin , Method validation
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